Shankar Mukherji

Titles
Assistant Professor

Office Contact Information

Degrees
Ph.D.
Office
209 Crow
Mailbox

Physics Department, CB 1105
Washington University
One Brookings Drive
St. Louis, MO 63130-4899, USA

Phone
(314) 935-5605

Research Interests

Arguably the grandest goal in cellular biophysics is the uncovering of design principles that govern all aspects of cellular function. Efforts in systems and synthetic cell biology have focused mainly on the design principles of gene expression and signaling systems. A quantitative understanding of eukaryotic cellular organization in space, however, would afford biophysicists and bioengineers with a powerful opportunity to predict how the physical architecture of the cell constrains and regulates fundamental life processes. To unleash this potential, it is imperative to understand one of the defining features of the eukaryotic cell: its organization into spatial compartments known as organelles. Coordinating organelle abundance and activity with developmental and environmental cues is one of the chief ways the cell can match its biochemical capabilities with its physiological demands. How does the cell orchestrate flows of matter and energy to produce exquisitely defined organelles at the nanometer and femtoliter scales of a cell? Can we engineer the decision-making processes in the cell to control organelle copy number, size, and composition in vivo and can this allow us to rationally alter cellular metabolism and signaling toward desired goals?

In our group, we aim to use a combination of theory and experiment to uncover the design principles that control:

  1. how the cell regulates organelle biogenesis
  2. how, in turn, organelles communicate with the rest of the cell to regulate cellular physiology

 

 

Selected Publications

  • Mukherji S, O’Shea EK (2014). Mechanisms of organelle biogenesis govern stochastic fluctuations in organelle abundance. eLife 3, e02678
  • Teng SW, Mukherji S, Moffitt JR, de Buyl S, O’Shea EK (2013). Robust circadian oscillations in growing cyanobacteria require transcriptional feedback. Science 340, 737-740.
  • Mukherji S, Ebert MS, Zheng G, Tsang JS, Sharp PA, van Oudenaarden A (2011). MicroRNAs can generate thresholds in target gene expression. Nature Genetics 43(9), 854-859.

Education

2004-2010 PhD, Massachusetts Institute of Technology/Harvard Medical School
2000-2004 SB (Physics), SB (Mathematics), Massachusetts Institute of Technology

 

Professional History

2017-present Assistant Professor, Washington University
2010-2017 Postdoctoral Fellow, Harvard University